Forced-Flow Layer Chromatography Forced-Flow Layer Chromatography (FFLC) provides readers with an update on the methods and techniques used in separation, a topic on which there are few devoted resources. Scientists in a variety of disciplines will find useful information on the many applications and developments that have taken place since the FFLC technique was first described by the authors nearly forty years ago. The book presents a number of variations of thin-layer chromatographic techniques, with special emphasis on overpressured-layer chromatography (OPLC) techniques and newer developments, such as the BioArena System for biomedical analysis. In addition, it takes a close look at the specifics of forced-flow layer chromatography, from the evolution of the methodology to the nuances of using these techniques in a variety of applications where traditional TLC is not as effective. The versatility of these forced flow techniques opens up new vistas for the analysis of a large number of samples (up to 216) for high throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis.
Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spotsEmphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years agoDiscusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances ISBN: 9780124201613, 012420161X
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